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The Induction of -Galactosidase in Escherichia coli Discuss

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The Induction of ß-Galactosidase in Escherichia coli
Discuss the results and explain their meaning in terms of the molecular regulation of the lac operon
lac Operon Practical
•You will outgrow E.colifrom minimal media after adding 3 supplemental food sources
: -Lactose
-Glucose + Lactose
-Water (nothing!)
•You will use a spectrophotometer to assess the effects by assaying for: ¾Light scattering (measures cell density) ¾β-Galactosidaseactivity (from Lac operon

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The Induction of -Galactosidase in Escherichia coli Discuss

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