Briefly explain the behavior observed in Plot III. (This trend persists beyond 24 h.)

Briefly explain the behavior observed in Plot III. (This trend persists beyond 24 h.)

116) the authors used flow cytom Show more In a recent paper (Islaih et al Mutation Research 578 (2005) 100116) the authors used flow cytometry to characterize DNA content in different cell populations. The data above shows DNA quantity per cell on the x-axis and number of cells on the y-axis. The control population received no modification. A second population was exposed to a 4-hour treatment of a weak mutagen hydrogen peroxide (H2O2). A third population was exposed to a 4-hour treatment of a strong mutagen Mitomycin-C. A. Is the control population actively engaged in the cell cycle or has it exited/paused in G0? (Choose ONE option below) B. The data for Plot II were taken 8 hours after hydrogen peroxide treatment. The data indicate that cell cycle arrest has occurred in: (choose ONE) C. Which protein or protein complex is primarily responsible for the cell cycle arrest observed in Plot II? (choose ONE) D. 24 hours after the hydrogen peroxide treatment the flow cytometry data look similar to the control again. Briefly explain this observation. E. The data for Plot III were taken 24 hours after Mitomycin-C treatment. The data indicated that cell cycle arrest has occurred in: (choose ONE) F. Briefly explain the behavior observed in Plot III. (This trend persists beyond 24 h.) Show less

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