LAB report 12 HOURS( 2 PAGES)
DO NOT DO RESULT SECTIONThis lab report will cover the transformation of E.coli and the restriction digest of the plasmid (labs 5 to 8).Use the Standard lab report format and include the following- Overall objective- Background to what you were trying to do- Methods – used to create reagents and used for the transformation, plasmid isolation, restriction digest and agarose gels. Create sub section for eachTransformation- What was the selectable marker?- How do you know you successfully transformed the cells with pGlo- What was the most critical step in the transformation processPlasmid preparation- Why does only the E. coli grown in ara/amp fluoresceRestriction digest- What size fragments would you expect when cutting the plasmid with the two enzymes used?- Are the results of the plasmid what you expected.- Are the two fragments the sizes you expected ?
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